Determine some of the virulent factors for isolates of Staphylococcus aureus by using a gene (icaA)

Abstract

Identifying the icaA gene in S. aureus isolates and assessing biofilm development using the PCR method were the goals of this investigation. The study included 20 S. aureus isolates sourced from patients with wound infections and other instances. The catalase, motility, coagulase, and growth on mannitol. We used salt agar tests to find S. aureus. We used the disc diffusion method to evaluate all S. aureus isolates for antibiotic resistance against four β-lactam drugs. Using the PCR approach, scientists found the icaA gene in S. aureus isolates. 90% of the isolates were icaA positive, while 10% were icaA negative. The analysis revealed that 60% of the S. aureus isolates had positive biofilm formation, whereas 40% displayed negative biofilm formation.